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產(chǎn)品簡(jiǎn)介

牛血清白蛋白（Bovine Serum Albumin，BSA），也稱(chēng)為第五組分（Cohn Fraction V），CAS NO. 9048-46-8，具有非常廣泛的生物學(xué)和診斷學(xué)研究用途。免疫實(shí)驗(yàn)中可用作封閉劑，功能類(lèi)似脫脂奶粉，動(dòng)物正常血清。也可用作載體蛋白，將其交聯(lián)于半抗原和其他弱抗原上，以提高抗體生產(chǎn)的免疫原性。酶和重組蛋白生產(chǎn)制品中加入BSA，不僅可以提高穩(wěn)定性，延長(zhǎng)保存周期；還能防止制品黏附到管壁或其他材料表面造成的損失。也常用于生物制藥的加工過(guò)程，或者作為營(yíng)養(yǎng)成分加入細(xì)胞和微生物培養(yǎng)體系中。還能用作蛋白定量檢測(cè)所需的定量標(biāo)準(zhǔn)品。

本品為陽(yáng)離子化牛血清白蛋白（Cationic Bovine Serum Albumin, cBSA），經(jīng)特殊的化學(xué)處理蛋白包含更多的胺基，使其在中性pH下有更多正電荷。cBSA是一種優(yōu)越的載體蛋白用來(lái)生產(chǎn)抗體，因其比未修飾BSA偶聯(lián)物具更強(qiáng)的免疫反應(yīng)性。cBSA還能用作免疫原來(lái)誘導(dǎo)免疫復(fù)合物引起的腎小球腎炎（ICGN）小鼠模型。本品以液體形式提供，濃度為2mg/ml。

保存與運(yùn)輸方法

保存：-20℃避光保存，1年有效。

注意事項(xiàng)

為了您的安全和健康，請(qǐng)穿實(shí)驗(yàn)服并戴一次性手套操作。

參考文獻(xiàn)

1、Bass PS, Drake AF, Wang Y, Thomas JH, Davies DR. Cationization of bovine serum albumin alters its conformation as well as its charge. Lab Invest. 1990 Feb;62(2):185-8. PMID: 2304331.

2、Debiec H, Lefeu F, Kemper MJ, Niaudet P, Deschênes G, Remuzzi G, Ulinski T, Ronco P. Early-childhood membranous nephropathy due to cationic bovine serum albumin. N Engl J Med. 2011 Jun 2;364(22):2101-10. PMID: 21631322.

3、Jin-Shuen Chen, Ann Chen, Li-Chien Chang, Wun-Shaing Wayne Chang, Herng-Sheng Lee, Shih-Hua Lin, Yuh-Feng Lin, Mouse model of membranous nephropathy induced by cationic bovine serum albumin: antigen dose–response relations and strain differences, Nephrology Dialysis Transplantation, Volume 19, Issue 11, November 2004, Pages 2721–2728.

4、Wu CC, Chen JS, Chen SJ, Lin SH, Chen A, Chang LC, Sytwu HK, Lin YF. Kinetics of adaptive immunity to cationic bovine serum albumin-induced membranous nephropathy. Kidney Int. 2007 Oct;72(7):831-40. doi: 10.1038/sj.ki.5002426. Epub 2007 Jul 11. PMID: 17622271.

以下來(lái)源于文獻(xiàn)資料（cBSA的制備方法，Ref, Mouse model of membranous nephropathy induced by cationic bovine serum albumin: antigen dose–response relations and strain differences）

Briefly, 200 g of crystallized bovine serum albumin was dissolved in 1.2 ml of distilled water. Anhydrous ethylenediamine (EDA) was prepared by mixing 2.68 ml of EDA and 20 ml of distilled water. The pH was adjusted to 4.75 with 6 N HCl and the solution cooled to 25°C. This EDA solution was added slowly to the BSA solution followed by 150 mg of 1-ethyl-3-[(3-dimethylaminopropyl)-carbodiimide hydrochloride] (EDC) with continuous stirring for 4 h. The product was purified using the pH-dependent binding technique. The reaction solution was applied to a 1 ml Econo Pac® High S cationic ion-exchange column (BioRad, Hercules, CA). After binding of BSA to the ion-exchange beads, the column was washed with 10 ml of pH 6.8 phosphate buffer (40 mM) to remove the non-reacted BSA. Subsequently, the modified BSA was eluted with 5 ml of 40 mM phosphate buffer (pH 10.6). All collections were then pooled, concentrated, and reconstituted with saline. Prior to use, the final protein concentration was measured using UV absorbance at 280 nm.